© 2003 |
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Department of Health and Human Services |
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National Institutes of Health |
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National Institute for Arthritis and Musculoskeletal and Skin Diseases |
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Center for Information Technology |
JOINSOLVER® Results Help |
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JOINSOLVER® Results |
| Summary |
Amino acid translation of the sequence:
QVQLVESGGGVVQPGRSLRLSCAASGFTFRSYAMHWVRQAPGKGLEWVAVISYDGTNKYYADSVKGRFTISRDNTKNTLYLQMNSLRTED
TAVYYCARDRETYGSGSYYKDQRTTTVWTSGAKGPRSPS
JoinSolver
Rearrangement is nonproductive:
JH is out of frame.
Highest scoring matches:
V: IGHV3-30*04 D:IGHD3-10*01 J: IGHJ6*02
The V segment has 6 mutations.
CDR3 Length: 71 A R D R E T Y G S G S Y Y K D Q R T T T V W T GCG AGA GAT CGC GAA ACC TAT GGT TCG GGG AGT TAT TAT AAA GAT CAG AGG ACC ACT ACG GTA TGG ACG TC
| V Match Results | ||
----------------------------------------------------------FR1----------- | ||
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 | ||
Q V Q L V E S G G G V V Q P G R S L | ||
score |
CAG GTG CAG CTG GTG GAG TCT GGG GGA GGC GTG GTC CAG CCT GGG AGG TCC CTG | |
IGHV3-30*04 | 1371 | ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... |
IGHV3-30*01 | 1362 | ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... |
IGHV3-30*09 | 1362 | ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... |
IGHV3-30*14 | 1362 | ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... |
IGHV3-30-3*01 | 1362 | ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... |
----------------------------------------------- ___________CDR1_________ | ||
19 20 21 22 23 24 25 26 27 28 29 30 31 31a 31b 32 33 34 | ||
R L S C A A S G F T F R S Y A M | ||
AGA CTC TCC TGT GCA GCC TCT GGA TTC ACC TTC AGA AGC --- --- TAC GCT ATG | ||
IGHV3-30*04 | ... ... ... ... ... ... ... ... ... ... ... ..T ... --- --- ..T ... ... | |
IGHV3-30*01 | ... ... ... ... ... ... ... ... ... ... ... ..T ... --- --- ..T ... ... | |
IGHV3-30*09 | ... ... ... ... ... ... ... ... ... ... ... ..T ... --- --- ..T ... ... | |
IGHV3-30*14 | ... ... ... ... ... ... ... ... ... ... ... ..T ... --- --- ..T ... ... | |
IGHV3-30-3*01 | ... ... ... ... ... ... ... ... ... ... ... ..T ... --- --- ..T ... ... | |
___ --------------------------------FR2-------------------- ____________ | ||
35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 | ||
H W V R Q A P G K G L E W V A V I S | ||
CAC TGG GTC CGC CAG GCT CCA GGC AAG GGG CTG GAG TGG GTG GCA GTT ATA TCA | ||
IGHV3-30*04 | ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... | |
IGHV3-30*01 | ... ... ... ... ... ... ... ... ... ... ..A ... ... ... ... ... ... ... | |
IGHV3-30*09 | ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... | |
IGHV3-30*14 | ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... | |
IGHV3-30-3*01 | ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... | |
________________________CDR2___________________________________ -------- | ||
52a 52b 52c 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 | ||
Y D G T N K Y Y A D S V K G R F | ||
TAT --- --- GAT GGA ACT AAT AAA TAT TAC GCA GAC TCC GTG AAG GGC CGA TTC | ||
IGHV3-30*04 | ... --- --- ... ... .G. ... ... ..C ... ... ... ... ... ... ... ... ... | |
IGHV3-30*01 | ... --- --- ... ... .G. ... ... ..C ... ... ... ... ... ... ... ... ... | |
IGHV3-30*09 | ... --- --- ... ... .G. ... ... ..C ... ... ... ... ... ... ... ... ... | |
IGHV3-30*14 | ... --- --- ... ... .G. ... ... ..C ... ... ... ... ... ... ... ... ... | |
IGHV3-30-3*01 | ... --- --- ... ... .GC ... ... ..C ... ... ... ... ... ... ... ... ... | |
------------------FR3--------------------------------------------------- | ||
68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 82a 82b 82c | ||
T I S R D N T K N T L Y L Q M N S L | ||
ACC ATC TCC AGA GAC AAT ACC AAG AAC ACG CTG TAT CTG CAA ATG AAC AGC CTG | ||
IGHV3-30*04 | ... ... ... ... ... ... T.. ... ... ... ... ... ... ... ... ... ... ... | |
IGHV3-30*01 | ... ... ... ... ... ... T.. ... ... ... ... ... ... ... ... ... ... ... | |
IGHV3-30*09 | G.. ... ... ... ... ... T.. ... ... ... ... ... ... ... ... ... ... ... | |
IGHV3-30*14 | ... ... ... ... ... ... T.. ... ... ... ... ... ..T ... ... ... ... ... | |
IGHV3-30-3*01 | ... ... ... ... ... ... T.. ... ... ... ... ... ... ... ... ... ... ... | |
-------------------------------------- ___CDR3_____ | ||
83 84 85 86 87 88 89 90 91 92 93 94 95 | ||
R T E D T A V Y Y C A R D | ||
AGA ACT GAG GAC ACG GCT GTG TAT TAC TGT GCG AGA GAT | ||
IGHV3-30*04 | ... G.. ... ... ... ... ... ... ... ... ... ... .. | |
IGHV3-30*01 | ... G.. ... ... ... ... ... ... ... ... ... ... .. | |
IGHV3-30*09 | ... G.. ... ... ... ... ... ... ... ... ... ... .. | |
IGHV3-30*14 | ... G.. ... ... ... ... ... ... ... ... ... ... .. | |
IGHV3-30-3*01 | ... G.. ... ... ... ... ... ... ... ... ... ... | |
| D Match Results | ||
A R D R E T Y G S G S Y Y K D Q R T T T V W T S G A K G P R S P S | ||
| len|rf | GCG AGA GAT CGC GAA ACC TAT GGT TCG GGG AGT TAT TAT AAA GAT CAG AGG ACC ACT ACG GTA TGG ACG TCT GGG GCC AAG GGA CCA CGG TCA CCG TCT CC | |
IGHD3-10*01 | 24|2 | G T.T TA. ... ... ... ... ... ... ... ..C |
IGHD3-10*02 | 19|1 | .T. TTA CTA T.. ... ... ... ... ... ..C |
IGHD3-9*01 | 10|2 | G T.T TA. G.. AT. .T. ACT G.. ... ... ..C |
IGHD3-16*02 | 10|2 | G TAT T.T GAT ..C .T. .G. ... ... ... CG. T.T ACC |
IGHD3-16*01 | 10|2 | G TAT T.T GAT ..C .T. .G. ... ... ... GC. T.T ACC |
| J Match Results | ||
A R D R E T Y G S G S Y Y K D Q R T T T V W T S G A K G P R S P S | ||
score | GCG AGA GAT CGC GAA ACC TAT GGT TCG GGG AGT TAT TAT AAA GAT CAG AGG ACC ACT ACG GTA TGG ACG TCT GGG GCC AAG GGA CCA CGG TCA CCG TCT CC | |
IGHJ6*02 | 223 | AT. ACT .CT ..T ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ..T CAG |
IGHJ6*01 | 214 | AT. ACT .CT ..T ... ... ... ... ... ... ... .G. ... ... ... ... ... ... ... ..T CAG |
IGHJ6*03 | 187 | AT. ACT .CT ..T ... ..T AC. ... ... ... ... ..A ... ... ... ... ... ... ... ..T CAG |
IGHJ5*01 | 145 | ..A ..T .GT .C. ..T C.. ... ... ... .A. ..C T.. ... ... ... ..T CAG |
IGHJ3*01 | 140 | TG .T. C.T .T. .T. ... ... ... ... ... .A. T.. ... ... ... .TT CAG |
V Match: IGHV3-30*04 D Match: IGHD3-10*01 J Match: IGHJ6*02 V-J Distance: 46 VD Junction: # of P,N Nucleotides: (2,7) (ga)tcGCGAAAC DJ Junction: # of P,N Nucleotides: (0,13) AGATCAGAGGACCMutation Analysis
Using Germline: IGHV3-30*04
A. Total number of VH bases through codon 92 : 288
B. Total Number of VH mutations : 6
C. Frequency of VH mutations: 2.08%
D. a) number of RGYW motifs: 11
b) number of mutations in RGYW motifs: 3
E. a) number of WRCY motifs: 9
b) number of mutations in WRCY motifs: 3
F. a) number of WA motifs: 25
b) number of mutations in WA motifs: 1
G. a) number of TW motifs: 19
b) number of mutations in TW motifs: 2
H. a)Total number of each base: G:88 C:69 A:67 T: 64
b) Number of mutated G: 2 C: 1 A: 0 T: 3
I. Number of base substitutions:
T>A: 2 T>G: 0 T>C: 1
A>T: 0 A>G: 0 A>C: 0
G>T: 0 G>A: 1 G>C: 1
C>T: 1 C>A: 0 C>G: 0
J. Number of transitions: 3
K. Number of transversions: 3
L. Amino Acid Replacements
1: 30 S>>R
2: 55 S>>T
3: 74 S>>T
4: 84 A>>T
M. R/S ratio:
FR Total: 3/0 CDR Total: 0.50
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